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human hepatoma cell line plc prf 5  (ATCC)


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    ATCC human hepatoma cell line plc prf 5
    In vitro evaluation of molnupiravir in HEV cell culture models. (A) Cytotoxicity of molnupiravir, ribavirin, and <t>sofosbuvir</t> <t>in</t> <t>PLC/PRF/5</t> cells and PRH. Three replicates per concentration. Bars represent the mean and SEM. Mean viability at each concentration was compared with the no-drug control group using the Student t test; ** indicates statistically significant difference ( p <0.05). (B) PRH infected with rHEV and treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. (C) Immunofluorescence staining for rHEV-infected PRH with/without antivirals: uninfected cells (negative control), rHEV-infected cells treated with either PBS (no drug control), sofosbuvir 10 μM, ribavirin 200 μM, or molnupiravir 100 μM. Cells were stained with polyclonal anti-rHEV sera and counterstained with DAPI. (D) PLC/PRF/5 cells infected with HEV infectious clone Kernow-p6, (E) PLC/PRF/5 cells infected with human-derived HEV. Cells were treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. Error bars represent SEM (n=3 replicates per concentration). Mean viral loads at each concentration were compared with the no-drug control group using the Student t test; * indicates statistically significant difference ( p <0.05) from the untreated mean. ** indicates an extremely statistically significant difference ( p <0.01) from the untreated mean. Abbreviations: HEV, hepatitis E virus; PRH, primary rat hepatocytes; SEM, standard error of the mean; PBS, phosphate-buffered saline; rHEV, Rocahepevirus ratti genotype 1; DAPI, 4′,6-diamidino-2-phenylindole.
    Human Hepatoma Cell Line Plc Prf 5, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Molnupiravir is effective against hepatitis E virus infection in an animal model"

    Article Title: Molnupiravir is effective against hepatitis E virus infection in an animal model

    Journal: Hepatology Communications

    doi: 10.1097/HC9.0000000000000944

    In vitro evaluation of molnupiravir in HEV cell culture models. (A) Cytotoxicity of molnupiravir, ribavirin, and sofosbuvir in PLC/PRF/5 cells and PRH. Three replicates per concentration. Bars represent the mean and SEM. Mean viability at each concentration was compared with the no-drug control group using the Student t test; ** indicates statistically significant difference ( p <0.05). (B) PRH infected with rHEV and treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. (C) Immunofluorescence staining for rHEV-infected PRH with/without antivirals: uninfected cells (negative control), rHEV-infected cells treated with either PBS (no drug control), sofosbuvir 10 μM, ribavirin 200 μM, or molnupiravir 100 μM. Cells were stained with polyclonal anti-rHEV sera and counterstained with DAPI. (D) PLC/PRF/5 cells infected with HEV infectious clone Kernow-p6, (E) PLC/PRF/5 cells infected with human-derived HEV. Cells were treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. Error bars represent SEM (n=3 replicates per concentration). Mean viral loads at each concentration were compared with the no-drug control group using the Student t test; * indicates statistically significant difference ( p <0.05) from the untreated mean. ** indicates an extremely statistically significant difference ( p <0.01) from the untreated mean. Abbreviations: HEV, hepatitis E virus; PRH, primary rat hepatocytes; SEM, standard error of the mean; PBS, phosphate-buffered saline; rHEV, Rocahepevirus ratti genotype 1; DAPI, 4′,6-diamidino-2-phenylindole.
    Figure Legend Snippet: In vitro evaluation of molnupiravir in HEV cell culture models. (A) Cytotoxicity of molnupiravir, ribavirin, and sofosbuvir in PLC/PRF/5 cells and PRH. Three replicates per concentration. Bars represent the mean and SEM. Mean viability at each concentration was compared with the no-drug control group using the Student t test; ** indicates statistically significant difference ( p <0.05). (B) PRH infected with rHEV and treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. (C) Immunofluorescence staining for rHEV-infected PRH with/without antivirals: uninfected cells (negative control), rHEV-infected cells treated with either PBS (no drug control), sofosbuvir 10 μM, ribavirin 200 μM, or molnupiravir 100 μM. Cells were stained with polyclonal anti-rHEV sera and counterstained with DAPI. (D) PLC/PRF/5 cells infected with HEV infectious clone Kernow-p6, (E) PLC/PRF/5 cells infected with human-derived HEV. Cells were treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. Error bars represent SEM (n=3 replicates per concentration). Mean viral loads at each concentration were compared with the no-drug control group using the Student t test; * indicates statistically significant difference ( p <0.05) from the untreated mean. ** indicates an extremely statistically significant difference ( p <0.01) from the untreated mean. Abbreviations: HEV, hepatitis E virus; PRH, primary rat hepatocytes; SEM, standard error of the mean; PBS, phosphate-buffered saline; rHEV, Rocahepevirus ratti genotype 1; DAPI, 4′,6-diamidino-2-phenylindole.

    Techniques Used: In Vitro, Cell Culture, Concentration Assay, Control, Infection, Immunofluorescence, Staining, Negative Control, Derivative Assay, Virus, Saline



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    In vitro evaluation of molnupiravir in HEV cell culture models. (A) Cytotoxicity of molnupiravir, ribavirin, and <t>sofosbuvir</t> <t>in</t> <t>PLC/PRF/5</t> cells and PRH. Three replicates per concentration. Bars represent the mean and SEM. Mean viability at each concentration was compared with the no-drug control group using the Student t test; ** indicates statistically significant difference ( p <0.05). (B) PRH infected with rHEV and treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. (C) Immunofluorescence staining for rHEV-infected PRH with/without antivirals: uninfected cells (negative control), rHEV-infected cells treated with either PBS (no drug control), sofosbuvir 10 μM, ribavirin 200 μM, or molnupiravir 100 μM. Cells were stained with polyclonal anti-rHEV sera and counterstained with DAPI. (D) PLC/PRF/5 cells infected with HEV infectious clone Kernow-p6, (E) PLC/PRF/5 cells infected with human-derived HEV. Cells were treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. Error bars represent SEM (n=3 replicates per concentration). Mean viral loads at each concentration were compared with the no-drug control group using the Student t test; * indicates statistically significant difference ( p <0.05) from the untreated mean. ** indicates an extremely statistically significant difference ( p <0.01) from the untreated mean. Abbreviations: HEV, hepatitis E virus; PRH, primary rat hepatocytes; SEM, standard error of the mean; PBS, phosphate-buffered saline; rHEV, Rocahepevirus ratti genotype 1; DAPI, 4′,6-diamidino-2-phenylindole.
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    In vitro evaluation of molnupiravir in HEV cell culture models. (A) Cytotoxicity of molnupiravir, ribavirin, and <t>sofosbuvir</t> <t>in</t> <t>PLC/PRF/5</t> cells and PRH. Three replicates per concentration. Bars represent the mean and SEM. Mean viability at each concentration was compared with the no-drug control group using the Student t test; ** indicates statistically significant difference ( p <0.05). (B) PRH infected with rHEV and treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. (C) Immunofluorescence staining for rHEV-infected PRH with/without antivirals: uninfected cells (negative control), rHEV-infected cells treated with either PBS (no drug control), sofosbuvir 10 μM, ribavirin 200 μM, or molnupiravir 100 μM. Cells were stained with polyclonal anti-rHEV sera and counterstained with DAPI. (D) PLC/PRF/5 cells infected with HEV infectious clone Kernow-p6, (E) PLC/PRF/5 cells infected with human-derived HEV. Cells were treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. Error bars represent SEM (n=3 replicates per concentration). Mean viral loads at each concentration were compared with the no-drug control group using the Student t test; * indicates statistically significant difference ( p <0.05) from the untreated mean. ** indicates an extremely statistically significant difference ( p <0.01) from the untreated mean. Abbreviations: HEV, hepatitis E virus; PRH, primary rat hepatocytes; SEM, standard error of the mean; PBS, phosphate-buffered saline; rHEV, Rocahepevirus ratti genotype 1; DAPI, 4′,6-diamidino-2-phenylindole.
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    In vitro evaluation of molnupiravir in HEV cell culture models. (A) Cytotoxicity of molnupiravir, ribavirin, and sofosbuvir in PLC/PRF/5 cells and PRH. Three replicates per concentration. Bars represent the mean and SEM. Mean viability at each concentration was compared with the no-drug control group using the Student t test; ** indicates statistically significant difference ( p <0.05). (B) PRH infected with rHEV and treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. (C) Immunofluorescence staining for rHEV-infected PRH with/without antivirals: uninfected cells (negative control), rHEV-infected cells treated with either PBS (no drug control), sofosbuvir 10 μM, ribavirin 200 μM, or molnupiravir 100 μM. Cells were stained with polyclonal anti-rHEV sera and counterstained with DAPI. (D) PLC/PRF/5 cells infected with HEV infectious clone Kernow-p6, (E) PLC/PRF/5 cells infected with human-derived HEV. Cells were treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. Error bars represent SEM (n=3 replicates per concentration). Mean viral loads at each concentration were compared with the no-drug control group using the Student t test; * indicates statistically significant difference ( p <0.05) from the untreated mean. ** indicates an extremely statistically significant difference ( p <0.01) from the untreated mean. Abbreviations: HEV, hepatitis E virus; PRH, primary rat hepatocytes; SEM, standard error of the mean; PBS, phosphate-buffered saline; rHEV, Rocahepevirus ratti genotype 1; DAPI, 4′,6-diamidino-2-phenylindole.

    Journal: Hepatology Communications

    Article Title: Molnupiravir is effective against hepatitis E virus infection in an animal model

    doi: 10.1097/HC9.0000000000000944

    Figure Lengend Snippet: In vitro evaluation of molnupiravir in HEV cell culture models. (A) Cytotoxicity of molnupiravir, ribavirin, and sofosbuvir in PLC/PRF/5 cells and PRH. Three replicates per concentration. Bars represent the mean and SEM. Mean viability at each concentration was compared with the no-drug control group using the Student t test; ** indicates statistically significant difference ( p <0.05). (B) PRH infected with rHEV and treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. (C) Immunofluorescence staining for rHEV-infected PRH with/without antivirals: uninfected cells (negative control), rHEV-infected cells treated with either PBS (no drug control), sofosbuvir 10 μM, ribavirin 200 μM, or molnupiravir 100 μM. Cells were stained with polyclonal anti-rHEV sera and counterstained with DAPI. (D) PLC/PRF/5 cells infected with HEV infectious clone Kernow-p6, (E) PLC/PRF/5 cells infected with human-derived HEV. Cells were treated with either PBS, varying concentrations of molnupiravir, ribavirin, and sofosbuvir, as well as combinations. Error bars represent SEM (n=3 replicates per concentration). Mean viral loads at each concentration were compared with the no-drug control group using the Student t test; * indicates statistically significant difference ( p <0.05) from the untreated mean. ** indicates an extremely statistically significant difference ( p <0.01) from the untreated mean. Abbreviations: HEV, hepatitis E virus; PRH, primary rat hepatocytes; SEM, standard error of the mean; PBS, phosphate-buffered saline; rHEV, Rocahepevirus ratti genotype 1; DAPI, 4′,6-diamidino-2-phenylindole.

    Article Snippet: Human hepatoma cell line PLC/PRF/5 (ATCC CRL-8024, LGC Standards, Wesel, Germany) and primary rat hepatocytes (PRH) were used in this study.

    Techniques: In Vitro, Cell Culture, Concentration Assay, Control, Infection, Immunofluorescence, Staining, Negative Control, Derivative Assay, Virus, Saline